| 產品編號 | bs-41304R |
| 英文名稱 | CD80 Rabbit pAb |
| 中文名稱 | 刺激分子B7-1蛋白抗體 |
| 別 名 | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costim |
| 研究領域 | 腫瘤 免疫學 干細胞 淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse CD80 protein: 38-246/306 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011] SWISS: Q00609 Gene ID: 12519 |
| 產品圖片 |
Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Human HL-60 cell lysates
Primary: Anti-CD80 (bs-41304R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 68 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag (Cat. bs-41304P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Mouse CD80 Antibody, the EC50 is 36.63 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag (Cat. bs-41304P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Mouse CD80 Antibody, the EC50 is 31.10 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
