| 產品編號 | bs-41372R |
| 英文名稱 | HES1 Rabbit pAb |
| 中文名稱 | 轉錄因子HES-1抗體 |
| 別 名 | bHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1(Drosophila); Hairy and enhancer of split 1; Hairy Enhancer of Split 1; Hairy homolog(Drosophila); Hairy Homolog; Hairy like; Hairy, Drosophila |
| 研究領域 | 免疫學 染色質和核信號 神經生物學 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產品應用 | WB=1:500-2000,ICC/IF=1:50-1:200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HES1 protein : 1-277/280 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. SWISS: Q14469 Gene ID: 3280 Database links: Entrez Gene: 395128 Chicken Entrez Gene: 3280 Human Entrez Gene: 15205 Mouse Omim: 139605 Human SwissProt: O57337 Chicken SwissProt: Q14469 Human SwissProt: P35428 Mouse Unigene: 250666 Human Unigene: 390859 Mouse Unigene: 19727 Rat |
| 產品圖片 |
Sample:
Lane 1: Recombinant human HES1 protein
Primary: Anti-HES1 (bs-41372R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Liver tissue lysates
Primary: Anti-HES1 (bs-41372R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 35 kDa
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HES1) polyclonal Antibody, unconjugated (bs-41372R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Measured by its binding ability in a indirect ELISA. Immobilized Human HES1 protein, His Tag (Cat. bs-41372P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human HES1 Antibody, the EC50 is 178.8 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
