| 產(chǎn)品編號 | bsm-60453R |
| 英文名稱 | CD23 Recombinant Rabbit mAb |
| 中文名稱 | CD23重組兔單抗 |
| 別 名 | Fc EpsilonR II; FcεRII; Blast 2; Blast2; C type lectin domain family 4 member J; C-type lectin domain family 4, member J; CD 23; CD 23A; CD23 antigen; CD23A; CLEC 4J; CLEC4J; Fc epsilon RII; Fc fragment of IgE low affinity II receptor for; Fc fragment of |
| 研究領域 | 細胞生物 免疫學 干細胞 細胞類型標志物 淋巴細胞 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | C2F9 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD23 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] Function: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). Subcellular Location: Cell membrane; Single-pass type II membrane protein. Secreted. Note=Also exists as a soluble excreted form. Post-translational modifications: N- and O-glycosylated. Similarity: Contains 1 C-type lectin domain. SWISS: P06734 Gene ID: 2208 Database links: Entrez Gene: 2208 Human Entrez Gene: 14128 Mouse Omim: 151445 Human SwissProt: P06734 Human SwissProt: P20693 Mouse Unigene: 465778 Human Unigene: 1233 Mouse CD23是一種II型跨膜糖蛋白，是存在于B細胞表面的IgE受體。在慢性B淋巴細胞白血病（CLL）和小B細胞淋巴瘤（SLL）為陽性，套細胞淋巴瘤和小核裂淋巴細胞淋巴瘤陰性。也表達于外周血B細胞的某些亞型、B淋巴細胞和EB病毒轉(zhuǎn)染的B淋巴瘤細胞系。CD23是慢性B淋巴細胞白血病，小B細胞淋巴瘤診斷和鑒別診斷有用的標記物。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-60453R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD23) Monoclonal Antibody, Unconjugated (bsm-60453R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
