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Mouse Anti-Cyclophilin B  antibody (bsm-33228M)  
~~~促銷代碼KT202411~~~
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產品編號 bsm-33228M
英文名稱 Mouse Anti-Cyclophilin B  antibody
中文名稱 親環蛋白PPIB(內參)單克隆抗體
別    名 Cphn 2; Cphn2; CyP 20b; CYP S1; CYP-S1; CYPB; MGC14109; MGC2224; peptidyl prolyl cis trans isomerase B; Peptidyl prolyl cis trans isomerase B precursor; Peptidyl-prolyl cis-trans isomerase B precursor; Peptidylprolyl isomerase B; PPIase; PPIB; Rotamase; S cyclophilin; SCYLP; PPIB_HUMAN.  
研究領域 腫瘤  細胞生物  免疫學  信號轉導  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 4C7
交叉反應 Human,Mouse,Rat
產品應用 WB=1:1000-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 20kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Cyclophilin B 
亞    型 IgG
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The cyclophilins are a conserved class of proteins that bind the immunosuppressive drug cyclosporin A (CsA) with high affinity. CsA blocks helper T-cell activation at a step between T-cell receptor stimulation and the transcriptional activation of cytokine genes. Cyclophilins from many species possess peptidyl-prolyl cis-trans isomerase (PPIase) activity that is blocked by CsA and therefore may be relevant in CsA-mediated immunosuppression.

Function:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

DISEASE:
Defects in PPIB are the cause of osteogenesis imperfect ype 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.

Similarity:
Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain.

SWISS:
P23284

Gene ID:
5479

Database links:

Entrez Gene: 5479 Human

Entrez Gene: 19035 Mouse

Entrez Gene: 64367 Rat

Omim: 123841 Human

SwissProt: P23284 Human

SwissProt: P24369 Mouse

SwissProt: P24368 Rat

Unigene: 434937 Human

Unigene: 335249 Mouse

Unigene: 1893 Rat



產品圖片
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: Thyroid gland (Mouse) Lysate at 40 ug Lane 5: Thyroid gland (Rat) Lysate at 40 ug Lane 6: Placenta (Mouse) Lysate at 40 ug Lane 7: Cerebrum (Mouse) Lysate at 40 ug Lane 8: Cerebrum (Rat) Lysate at 40 ug Lane 9: Liver (Mouse) Lysate at 40 ug Lane 10: Liver (Rat) Lysate at 40 ug Lane 11: Kidney (Mouse) Lysate at 40 ug Lane 12: Kidney (Rat) Lysate at 40 ug Lane 13: Huvec (Human) Cell Lysate at 30 ug Lane 14: U937 (Human) Cell Lysate at 30 ug Primary: Anti-Cyclophilin B (bsm-33228M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin B(Loading Control) Monoclonal Antibody, Unconjugated(bsm-33228M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclophilin B(Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclophilin B(Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclophilin B(Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclophilin B(Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33228M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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