| 產品編號 | bs-18994R |
| 英文名稱 | NAB1 Rabbit pAb |
| 中文名稱 | EGR1結合蛋白1抗體 |
| 別 名 | EGR 1 binding protein 1; EGR-1-binding protein 1; EGR1 binding protein 1; NAB1; NAB1_HUMAN; NGFI A binding protein 1(EGR1 binding protein 1); NGFI A binding protein 1; NGFI-A-binding protein 1; Transcriptional regulatory protein p54. |
| 研究領域 | 細胞生物 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NAB1: 401-487/487 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Transcriptional control is in part regulated by interactions between DNA-bound transcription factors, such as Egr1/NGFI-A, and coregulatory proteins, such as NAB (for NGFI-A-binding proteins). The evolutionarily conserved NAB proteins, NAB1 and NAB2, are corepressors of Egr1/NGFI-A. Both NAB1 and NAB2 contain an amino-terminal NAB-conserved domain 1 (NCB1), which is required for binding NGFI-A, and a carboxy-terminal NCD2, which is responsible for the repressor function of NAB proteins. NAB1 requires NGFI-A to gain access to DNA, indicating that NAB1 is an active repressor that works by a direct mechanism. NAB1, which is constitutively expressed, is localized exclusively in the nucleus and may play a role in controlling processes such as cell division, differentiation and apoptosis. Function: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Subcellular Location: Nucleus. Tissue Specificity: Isoform Short is found in a myeloid leukemia cell line. Similarity: Belongs to the NAB family. SWISS: Q13506 Gene ID: 4664 Database links: Entrez Gene: 4664 Human Entrez Gene: 17936 Mouse Omim: 600800 Human SwissProt: Q13506 Human SwissProt: Q61122 Mouse Unigene: 723892 Human Unigene: 25903 Mouse Unigene: 10099 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
