| 產品編號 | bs-14443R |
| 英文名稱 | Syntaxin 12 Rabbit pAb |
| 中文名稱 | 神經突觸素12抗體 |
| 別 名 | MGC51957; Stx12; STX12_HUMAN; STX13; STX14; Syntaxin-12. |
| 研究領域 | 神經生物學 信號轉導 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Syntaxin 12: 201-300/376 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Syntaxins, a family of proteins involved in the fusion of synaptic vesicles with the plasma membrane, display broad tissue distribution and contain C-terminal hydrophobic domains that direct them to their respective intracellular compartments. Syntaxin 12, also known as STX12, STX13 or STX14, is a 276 amino acid single-pass membrane protein that contains one t-SNARE coiled-coil homology domain and belongs to the Syntaxin family. Syntaxin 12 regulates protein transport between late endosomes and the trans-Golgi network and interacts with ABC1 (ATP-binding cassette transporter A1), a protein that facilitates cellular release of choline-phospholipids and cholesterol to apoA-I (apolipoprotein A-I). Found in recycling endosomes, Syntaxin 12 colocalizes with transferrin receptor and is encoded by a gene located on human chromosome 1. Function: SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. Subcellular Location: Membrane. Golgi apparatus > trans-Golgi network membrane. Similarity: Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain. SWISS: Q86Y82 Gene ID: 23673 Database links: Entrez Gene: 100226 Mouse SwissProt: Q9ER00 Mouse SwissProt: Q86Y82 Human Unigene: 28237 Mouse Unigene: 54570 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syntaxin 12) Polyclonal Antibody, Unconjugated (bs-14443R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
araformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syntaxin 12) Polyclonal Antibody, Unconjugated (bs-14443R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
