| 產品編號 | bs-13055R |
| 英文名稱 | EEF1D Rabbit pAb |
| 中文名稱 | 延伸因子EF-1δ抗體 |
| 別 名 | Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; Eukaryotic translation elongation factor 1 delta; Guanine nucleotide exchange protein. |
| 研究領域 | 細胞生物 轉錄調節因子 轉運蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EEF1D: 1-100/281 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a subunit of the elongation factor-1complex, which is responsible for the enzymatic delivery ofaminoacyl tRNAs to the ribosome. This subunit, delta, functions asguanine nucleotide exchange factor. It is reported that followingHIV-1 infection, this subunit interacts with HIV-1 Tat. Thisinteraction results in repression of translation of host cellproteins and enhanced translation of viral proteins. Severalalternatively spliced transcript variants encoding multipleisoforms have been found for this gene. Related pseudogenes havebeen defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided byRefSeq, Aug 2010]. Function: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Subunit: EF-1 is composed of 4 subunits: alpha, beta, deltaisoform 1, and gamma. Isoform 2 interacts with HSF1 and NFE2L2. Subcellular Location: Isoform 2: Nucleus. Tissue Specificity: Isoform 2 is specifically expressed in brain,cerebellum and testis. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the EF-1-beta/EF-1-delta family. SWISS: P29692 Gene ID: 1936 Database links: Entrez Gene: 1936 Human SwissProt: P29692 human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EEF1D) Polyclonal Antibody, Unconjugated (bs-13055R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
