| 產品編號 | bs-2589R |
| 英文名稱 | CYP1A2 Rabbit pAb |
| 中文名稱 | CYP1A2抗體 |
| 別 名 | Cytochrome P450 1A2; cytochrome P450, family 1, subfamily a, polypeptide 2; Cytochrome P450 family 1 polypeptide 2; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2; Cyp1a2; CYPD45; P-450d; RATCYPD45; CP12; CP 12; P3-450; Aryl hydroca |
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Specific References (5) | bs-2589R has been referenced in 5 publications.
[IF=5.923] Hanyi Hua. et al. The Intervention and Mechanism of Action for Aloin against Subchronic Aflatoxin B1 Induced Hepatic Injury in Rats. Int J Mol Sci. 2021 Jan;22(21):11620 IHC ; Rat.
[IF=4.15] Jiang, Zhihui, et al. "Development of an IgY Antibody-Based Immunoassay for the Screening of the CYP2E1 Inhibitor/Enhancer from Herbal Medicines." Frontiers in Pharmacology 7 (2016): 502. WB ; Mouse.
[IF=3.54] Huang, Li, et al. "Reduction of Aflatoxin B1 Toxicity by Lactobacillus plantarum C88: A Potential Probiotic Strain Isolated from Chinese Traditional Fermented Food “Tofu”." PLOS ONE 12.1 (2017): e0170109. WB ; Mouse.
[IF=2.85] Cui, Yan, et al. "Evaluating the hepatoprotective efficacy of Aloe vera polysaccharides against subchronic exposure of aflatoxins B 1." Journal of the Taiwan Institute of Chemical Engineers (2017). IHC-P ; Mouse.
[IF=1.785] Hongfang Li. et al. Effects of Xiaochaihu decoction on the expression of cytochrome P450s in rats. Exp Ther Med. 2021 Jun;21(6):1-9 WB ; Rat.
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| 研究領域 | 細胞生物 神經生物學 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP1A2: 221-320/513 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu region. [provided by RefSeq].sequences flanked by direct repeats in the 3' untranslated. Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Tissue Specificity: Liver. Similarity: Belongs to the cytochrome P450 family. SWISS: P05177 Gene ID: 1544 Database links: Entrez Gene: 1544 Human Entrez Gene: 13077 Mouse Omim: 124060 Human SwissProt: P05177 Human SwissProt: P00186 Mouse Unigene: 1361 Human Unigene: 15537 Mouse Unigene: 5563 Rat 合成與降解（Synthesis and Degradation） |
| 產品圖片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-P450 1A2 (bs-2589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P450 1A2 Polyclonal Antibody, Unconjugated(bs-2589R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-P450 1A2 antibody (bs-2589R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
