| 產(chǎn)品編號(hào) | bs-1851R |
| 英文名稱 | MKP1 Rabbit pAb |
| 中文名稱 | 絲裂原活化蛋白激酶磷酸酶-1抗體 |
| 別 名 | DUSP1; Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP 1; DUSP-1; EC 3.1.3.16; EC 3.1.3.48; HVH 1; HVH1; MAP kinase phos |
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Specific References (5) | bs-1851R has been referenced in 5 publications.
[IF=6.244] Meng-ke Fan. et al. Siglec-15 Promotes Tumor Progression in Osteosarcoma via DUSP1/MAPK Pathway. Front Oncol. 2021; 11: 710689 WB ; Human.
[IF=5.008] Kato, Hiroyuki, et al. "Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis." Oncotarget 7.2 (2016): 2009. IF(IHC-F) ; Rat.
[IF=4.522] Sha J et al. Dexmedetomidine improves acute stress‐induced liver injury in rats by regulating MKP‐1, inhibiting NF‐κB pathway and cell apoptosis. J Cell Physiol. 2019 Aug;234(8):14068-14078. IHF ; Rat.
[IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653 WB ; Human.
[IF=3.585] Sha J et al. Dexmedetomidine attenuates lipopolysaccharide-induced liver oxidative stress and cell apoptosis in rats by increasing GSK-3β/MKP-1/Nrf2 pathway activity via the α2 adrenergic receptor. Toxicol Appl Pharmacol. 2019 Feb 1;364:144-152. WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DUSP1: 151-250/367 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Function: Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'. Tissue Specificity: Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. Post-translational modifications: Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain. SWISS: P28562 Gene ID: 1843 Database links: Entrez Gene: 1843 Human Entrez Gene: 19252 Mouse Omim: 600714 Human SwissProt: P28562 Human SwissProt: P28563 Mouse Unigene: 171695 Human Unigene: 239041 Mouse Unigene: 98260 Rat MKPs是一類絲氨酸/蘇氨酸和酪氨酸雙重底物特異性的磷酸酶 ,對(duì)于絲裂素活化蛋白激酶活性的調(diào)節(jié)起著十分重要的作用，可使絲裂素活化蛋白激酶上的蘇氨酸/酪氨酸去磷酸化失活。目前研究發(fā)現(xiàn)MKPs分別有MKP-1、MKP-2、MKP-3及MKP4-6。 MKPs受MAPK信號(hào)通路中多種成分的誘導(dǎo) ,決定了它與MAPK之間作用的特異性。 通過(guò)去磷酸化作用調(diào)節(jié)MAPK信號(hào)途徑的活性 ,確保了細(xì)胞內(nèi)信號(hào)的精確傳遞 ,參與了多種主要的細(xì)胞功能的調(diào)節(jié)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HUVEC cell lysates
Primary: Anti-MKP1 (bs-1851R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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