| 產品編號 | bs-1566R |
| 英文名稱 | CRIPTO Rabbit pAb |
| 中文名稱 | 畸胎瘤衍化生長因子抗體(N端) |
| 別 名 | CRIPTO; CRIPT; CR; CRGF; Cripto 1; Cripto-1; Cripto 1 growth factor; Cripto-1 growth factor; Cripto1; Cripto1 growth factor; Epidermal growth factor like cripto protein CR1; Epidermal growth factor-like cripto protein CR1; TDGF 1; TDGF-1; TDGF1; Teratocar |
| 研究領域 | 腫瘤 信號轉導 細胞凋亡 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TDGF1: 31-150/188 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]. Function: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Subunit: Interacts with the activin type-1 receptor ACVR1B. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung. Similarity: Contains 1 EGF-like domain. SWISS: P13385 Gene ID: 6997 Database links: Entrez Gene: 6997 Human Entrez Gene: 21667 Mouse Omim: 187395 Human SwissProt: P13385 Human SwissProt: P51865 Mouse Unigene: 385870 Human Unigene: 5090 Mouse Cripto-1是表皮生長因子-Cripto-FRL-Criptic(EGF-CFC)家族成員之一, 屬于轉化生長細胞TGF-β蛋白家族及Nodal蛋白質的共同受體。Cripto蛋白對于胚胎的發育占有非常重要的地位。 Cripto-1在人類的多種腫瘤如結腸癌、乳腺癌、卵巢癌、胰腺癌等中高表達，可激活P13K/Akt/GSK-3βc-Src和ras/raf/MAPK信號通路,介導細胞增殖和抑制細胞凋亡，Cripto蛋白的單細胞繁殖抗體，可以有效抑制一些癌細胞的生長。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRIPTO) Polyclonal Antibody, Unconjugated (bs-1566R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRIPTO) Polyclonal Antibody, Unconjugated (bs-1566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRIPTO) Polyclonal Antibody, Unconjugated (bs-1566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRIPTO) Polyclonal Antibody, Unconjugated (bs-1566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
