| 产品编号 | bs-0879R |
| 英文名称 | Rabbit Anti-FLAG Tag (CT) antibody |
| 中文名称 | FLAG Tag标签（C端）抗体 |
| 别 名 | DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag. |
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Specific References (4) | bs-0879R has been referenced in 4 publications.
[IF=5.714] Mengmeng Zhao. et al. Interferon inducible porcine 2′, 5′-oligoadenylate synthetase like-1 protein limits porcine reproductive and respiratory syndrome virus 2 infection via the MDA5-mediated interferon-signaling pathway. INT IMMUNOPHARMACOL. 2022 Oct;111:109151 IP, WB ; Monkey, Pig.
[IF=5.52] Zhang, Zhenfeng, et al. "Human Bocavirus NP1 Inhibits IFN-?? Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter."The Journal of Immunology 189.3 (2012): 1144-1153.
[IF=3.636] Mengmeng Zhao. et al. Molecular cloning and functional analysis of Macaca mulatta STING. Dev Comp Immunol. 2022 Feb;127:104296 WB ; Monkey.
[IF=3.412] Mengmeng Zhao. et al. Sequence Analysis of Macaca mulatta TRIM4 and Its Role in the Interferon Pathway. Front Vet Sci. 2022; 9: 805301 WB ; Monkey.
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| 产品类型 | 标签抗体 |
| 研究领域 | 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:5000-20000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Flag Tag peptide |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. SWISS: N/A Gene ID: N/A 蛋白质纯化的新途径─应用抗DYKDDDDK标签8肽抗体检测DYKDDDDK标签N端8肽融合蛋白 1） DYKDDDDK标签8肽与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变性的条件下可进行进一步纯化。 2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） DYKDDDDK标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作?？捎糜谌魏伪泶锵低常òǎ?细菌，杆状病毒和哺乳动物） 4） DYKDDDDK8肽标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能，不会影响蛋白分泌 5） DYKDDDDK8肽标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。 |
| 产品图片 |
Sample: Lane1:DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-FLAG Tag (bs-0879R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
