| 產(chǎn)品編號(hào) | bs-1120R |
| 英文名稱 | FLIP Rabbit pAb |
| 中文名稱 | FLIP抗體 |
| 別 名 | CASP8 and FADD-like apoptosis regulator subunit p12; c FLIP; c FLIPL; c FLIPR; c FLIPS; CASH; CASP8 and FADD like regulator; CASP8 and FADD like apoptosis regulator precursor; CASP8AP1; Caspase Eight Related Protein; Caspase homolog; Caspase Homologue; Ca |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12/52 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CASP8 and FADD-like apoptosis regulator subunit p12 (Potential): 401-480/480 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]. Function: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. Tissue Specificity: Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Post-translational modifications: Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. Similarity: Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. SWISS: O15519 Gene ID: 8837 Database links: Entrez Gene: 8837 Human Entrez Gene: 12633 Mouse Omim: 603599 Human SwissProt: O15519 Human SwissProt: O35732 Mouse Unigene: 390736 Human Unigene: 336848 Mouse FLIP參與凋亡的調(diào)節(jié)。此抗體在長(zhǎng)型和短型的FLIP異構(gòu)體中均表達(dá)。短型FLIP包含2個(gè)死亡效應(yīng)基因結(jié)構(gòu)區(qū)，同源于FAS相關(guān)蛋白死亡效應(yīng)基因結(jié)構(gòu)區(qū)。長(zhǎng)型FLIP包含1個(gè)附加的Caspase 樣結(jié)構(gòu)區(qū)，但是他缺少一個(gè)催化部位和在大多數(shù)Caspase 蛋白中形成底物結(jié)合束的殘基。 |
| 產(chǎn)品圖片 |
Sample:
Heart(Mouse) Cell Lysate at 40 ug
Primary: Anti-FLIP (bs-1120R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/52 kD
Observed band size: 52 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-FLIP (bs-1120R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/52 kD
Observed band size: 52 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLIP Polyclonal Antibody, Unconjugated(bs-1120R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLIP Polyclonal Antibody, Unconjugated(bs-1120R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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